NAD+ Injection Dosage Guide: Protocols, Titration and How to Use

NAD+ (nicotinamide adenine dinucleotide) is a coenzyme studied for its role in cellular energy and metabolic pathways. As an injectable, it ships as a freeze-dried (lyophilized) powder that has to be reconstituted with bacteriostatic water before any volume can be measured. Subcutaneous protocols are discussed in much smaller amounts than the gram-scale IV drips you may have read about, which changes the math and the titration approach.

Everything below is third-person reference information for research and educational use. It is not dosing advice, and NAD+ is not approved for human consumption. Any decision about whether or how much to use belongs with a licensed clinician. To run your own concentration and unit numbers instead of the worked examples here, use the peptide dosage calculator.

Subcutaneous NAD+ dosing ranges

IV NAD+ is often referenced at 250 to 1000 mg per session because the slow drip spreads the load over hours. Subcutaneous protocols described in the literature use far smaller per-injection figures, commonly in a 20 to 100 mg range, precisely because the compound goes in faster than a drip and a large bolus is associated with strong flushing sensations. A 50 mg figure sits in the middle of that band and is a common reference point.

• Low reference band: 20 to 50 mg per injection
• Mid reference band: 50 to 100 mg per injection
• Frequency in protocols: often discussed as every other day or a few times per week, not daily

Why protocols start low

NAD+ is well known in user reports and research notes for a flushing, warmth, or pressure sensation when injected quickly, and that sensation scales with both the amount and the injection speed. This is the main reason titration protocols open at the bottom of the range. Starting at a small figure and increasing in steps lets the math stay conservative while the response is observed.

• A common pattern is to begin near 10 to 20 mg and step up gradually over weeks
• Slower injection speed is frequently described as reducing the flushing sensation independent of the amount
• Splitting a target figure across two smaller injections is another structure seen in protocols

None of these patterns are recommendations. They describe how titration is structured in reference material so the arithmetic below has context.

The reconstitution math

NAD+ injectable vials are often larger than peptide vials, with 100 mg, 500 mg, and 1000 mg sizes all in circulation. The same three-step logic applies: concentration sets everything, then two conversions follow.

• Concentration = vial strength / water volume. A 500 mg vial in 5 mL gives 100 mg/mL.
• Draw volume = dose / concentration. A 50 mg figure at 100 mg/mL is 0.5 mL.
• Units = draw volume x 100 on a U-100 insulin syringe. 0.5 mL is 50 units.

Because NAD+ figures are larger than typical peptide microgram figures, draw volumes can climb toward the top of a 0.5 mL or 1 mL syringe. Adding more water lowers the concentration and the unit count per milligram but raises the liquid volume per draw, so the two trade against each other. The reconstitution calculator handles the same arithmetic for any vial size.

Worked example: a 500 mg vial in 5 mL

A 500 mg vial reconstituted with 5 mL of bacteriostatic water gives 100 mg/mL, a clean reference setup because milligrams and units line up neatly. At this concentration the conversions are:

• 20 mg = 0.20 mL = 20 units
• 50 mg = 0.50 mL = 50 units
• 100 mg = 1.00 mL = 100 units

At 100 mg/mL a 500 mg vial holds 10 reference doses of 50 mg, or 25 at 20 mg. If you change the vial size, the water volume, or the target figure, recompute from scratch rather than reusing an old unit count. The mg to units converter isolates the final mass-to-units step if that is all you need.

Stacking notes with MOTS-c

NAD+ and MOTS-c are sometimes grouped together in metabolic-focused reference protocols because both are discussed in the context of mitochondrial pathways, though they act through different mechanisms and the figures are not interchangeable. MOTS-c is dosed in milligrams too but on a different scale, often referenced near 5 to 10 mg per injection a few times weekly. See the MOTS-c dosage guide for that compound's specific math.

• The two are kept in separate syringes in most stack references, since combining peptides in one barrel is a separate question covered in can you mix two peptides in one syringe
• Injection days are sometimes staggered rather than stacked on the same day to keep the response to each compound distinguishable
• Each compound keeps its own reconstitution math; a shared vial of bacteriostatic water does not mean a shared concentration

Handling and storage notes

General references describe reconstituted NAD+ as kept refrigerated and shielded from light, since it is sensitive to degradation. Bacteriostatic water (saline with benzyl alcohol) is the usual diluent for multi-draw vials because the preservative limits microbial growth between draws. Aim the water down the vial wall rather than onto the powder, and swirl rather than shake. For a fuller treatment, see how to store peptides.